In a previous work, we in silico annotated protein sequences of Citrus genus plants as\nputative tryptophan decarboxylase (pTDC). Here, we investigated the structural properties of Citrus\npTDCs by using the TDC sequence of Catharanthus roseus as an experimentally annotated reference\nto carry out comparative modeling and substrate docking analyses. The functional annotation as\nTDC was verified by combining 3D molecular modeling and docking simulations, evidencing the\npeculiarities and the structural similarities with C. roseus TDC. Docking with L-tryptophan as a ligand\nshowed specificity of pTDC for this substrate. These combined results confirm our previous in silico\nannotation of the examined protein sequences of Citrus as TDC and provide support for TDC activity\nin this plant genus.
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